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differentiation 36  (R&D Systems)


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    Structured Review

    R&D Systems differentiation 36
    Differentiation 36, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+cd36/Recombinant+Human+CD36%2FSR-B3+Fc+Chimera+Protein%2C+CF/pmc13064270-68-11-24
    Average 94 stars, based on 13 article reviews
    differentiation 36 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium -infected erythrocytes
    Article Snippet: .. For up-selection of CD36-binding Pf EMP1 variants, recombinant human CD36 (125 μg ml −1 in PBS, R&D Systems) was first immobilized on plastic Petri dishes. ..

    Article Title: Plasmodium falciparum FIKK Kinase Members Target Distinct Components of the Erythrocyte Membrane
    Article Snippet: .. Briefly, plastic Petri dishes were coated overnight at 4°C with phosphate-buffered saline (PBS) containing 1 mg/ml CSA sodium salt from bovine trachea (Sigma), 1 mg/ml chondroitin sulfate C sodium salt from shark cartilage (Sigma), 10 μg/ml recombinant human CD36 (R&D Systems). ..

    Article Title: Beninese children with cerebral malaria do not develop humoral immunity against the IT4-VAR19-DC8 PfEMP1 variant linked to EPCR and brain endothelial binding
    Article Snippet: .. Briefly, petri dishes (Falcon 1058) with 0.5-cm diameter spots were coated overnight at 4 °C with either 10 μl of PBS containing 50 μg/ml recombinant human CD36 (R&D Systems), 50 μg/ml recombinant human ICAM-1 (R&D Systems), 1 mg/ml CSA sodium salt from bovine trachea (Sigma), 50 μg/ml recombinant EPCR or 1 % BSA. ..

    Article Title: Plasmodium falciparum FIKK Kinase Members Target Distinct Components of the Erythrocyte Membrane
    Article Snippet: IEs were selected on the human placental derived BeWo cell line (European Collection of Cell Cultures) to obtain the FCR3-CSA parasite line or on Chinese Hamster Ovary cells-745 (CHO-745; American Type Culture Collection) expressing CD36 to obtain the FCR3-CD36 parasite line. .. Pannings were repeated three times, and parasites were tested for their ability to bind purified CSA (Sigma) or recombinant human CD36 (R&D Systems). ..

    Article Title: From In Vivo to In Vitro : Dynamic Analysis of Plasmodium falciparum var Gene Expression Patterns of Patient Isolates during Adaptation to Culture
    Article Snippet: .. Plastic Petri dishes were coated overnight at 4°C with 1xPBS containing either 1 mg/ml CSA sodium salt from bovine trachea (Sigma), 100 μg/ml HA sodium salt from bovine vitreous humor (Sigma), 10 μg/ml recombinant human CD36 (R&D Systems), 10 μg/ml recombinant human ICAM-1 (R&D Systems) or 1% BSA. .. These receptors were spotted onto a 150 mm petri dish (BD Falcon) in duplicates and incubated in a humid box overnight at 4°C.

    other:

    Article Title: Salvianolic acid B inhibits macrophage uptake of modified low density lipoprotein (mLDL) in a scavenger receptor CD36-dependent manner
    Article Snippet: The recombinant human CD36 (R&D, Minneapolis, MN) was diluted to 50 μg/mL in 10 mM sodium acetate (pH 4.5) and immobilized on a CM5 sensor chip by amino coupling at a total signal of 8,000 response units (RU).

    Saline:

    Article Title: Plasmodium falciparum FIKK Kinase Members Target Distinct Components of the Erythrocyte Membrane
    Article Snippet: .. Briefly, plastic Petri dishes were coated overnight at 4°C with phosphate-buffered saline (PBS) containing 1 mg/ml CSA sodium salt from bovine trachea (Sigma), 1 mg/ml chondroitin sulfate C sodium salt from shark cartilage (Sigma), 10 μg/ml recombinant human CD36 (R&D Systems). ..

    Inhibition:

    Article Title: Human C1-Inhibitor Suppresses Malaria Parasite Invasion and Cytoadhesion via Binding to Parasite Glycosylphosphatidylinositol and Host Cell Receptors
    Article Snippet: .. Inhibition of the binding was performed by preincubation of fluorescent-labeled C1INH with soluble recombinant human CD36 (R&D Systems) or soluble CSA (Sigma) at 100 μg/mL for 30 minutes at 37°C before incubation with CHO cells. .. Twenty female C57BL6 mice aged 8–10 weeks from Jackson Laboratories were infected with transgenic Plasmodium berghei strain ANKA expressing luciferase/green fluorescent protein under a constitutive promoter [ 31 ], and blood samples were collected on day 5 after infection.

    Binding Assay:

    Article Title: Human C1-Inhibitor Suppresses Malaria Parasite Invasion and Cytoadhesion via Binding to Parasite Glycosylphosphatidylinositol and Host Cell Receptors
    Article Snippet: .. Inhibition of the binding was performed by preincubation of fluorescent-labeled C1INH with soluble recombinant human CD36 (R&D Systems) or soluble CSA (Sigma) at 100 μg/mL for 30 minutes at 37°C before incubation with CHO cells. .. Twenty female C57BL6 mice aged 8–10 weeks from Jackson Laboratories were infected with transgenic Plasmodium berghei strain ANKA expressing luciferase/green fluorescent protein under a constitutive promoter [ 31 ], and blood samples were collected on day 5 after infection.

    Incubation:

    Article Title: Human C1-Inhibitor Suppresses Malaria Parasite Invasion and Cytoadhesion via Binding to Parasite Glycosylphosphatidylinositol and Host Cell Receptors
    Article Snippet: .. Inhibition of the binding was performed by preincubation of fluorescent-labeled C1INH with soluble recombinant human CD36 (R&D Systems) or soluble CSA (Sigma) at 100 μg/mL for 30 minutes at 37°C before incubation with CHO cells. .. Twenty female C57BL6 mice aged 8–10 weeks from Jackson Laboratories were infected with transgenic Plasmodium berghei strain ANKA expressing luciferase/green fluorescent protein under a constitutive promoter [ 31 ], and blood samples were collected on day 5 after infection.

    Purification:

    Article Title: Plasmodium falciparum FIKK Kinase Members Target Distinct Components of the Erythrocyte Membrane
    Article Snippet: IEs were selected on the human placental derived BeWo cell line (European Collection of Cell Cultures) to obtain the FCR3-CSA parasite line or on Chinese Hamster Ovary cells-745 (CHO-745; American Type Culture Collection) expressing CD36 to obtain the FCR3-CD36 parasite line. .. Pannings were repeated three times, and parasites were tested for their ability to bind purified CSA (Sigma) or recombinant human CD36 (R&D Systems). ..



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    Role of <t>CD36</t> in the clinical prognosis and immune infiltration correlation in cutaneous melanoma: ( A ) Hallmark enrichment plot of CD36 generated with core cancer hallmark gene set ( n = 1574) shows that CD36 is associated with invasion and metastasis of melanoma as well as reprogramming energy metabolism ( p < 0.05). ( B ) Progression-free survival with CD36 expression in melanoma patients, using upper (red) and lower (blue) quartile data from the SKCM dataset. ( C ) Correlation of CD36 with CD163, CD14, CD209, and FAP in SKCM dataset ( n = 471). ( D ) CD36 positively correlates with the infiltration level of M2 macrophages in the SKCM-metastasis dataset ( n = 368). ( E ) Correlation between CD36 and infiltration level of endothelial cells in SKCM-metastasis dataset ( n = 368).
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    Image Search Results


    Identification of the scFv anti-CD36 D11 (A) Strategy of panning of the ALTHEA Gold Libraries™ for identification of anti-CD36 scFvs. (B) Phage ELISA for 90 randomly selected clones from the third round of panning. Graph shows the signals generated by binding to rhCD36 (red) or protein L (ProL; brown). As control, we detected rhCD36 with the commercial antibody JC63.1 (blue). (C) Determination of structural integrity of purified scFv D11 by denaturing SDS-PAGE. R, reducing condition; NR, non-reducing condition; MW, molecular weight. (D, E) ELISA assay evaluating the binding of purified D11 (green) to protein L (D) or rhCD36 (E) . JC63.1 (blue) was used as a positive control and specificity was assessed by using the unrelated scFv P5E1A6 (black). (F) Representative competition ELISA (from two performed) evaluating the binding to rhCD36 captured by the reference antibody JC63.1 in presence of D11 (green) or the specificity control P5E1A6 (black). The signal generated in the absence of D11 is shown in red. (G) Number of clones identified at each stage of the discovery process. (G) was created with Biorender.com .

    Journal: Frontiers in Immunology

    Article Title: Discovery and in vitro characterization of a human anti-CD36 scFv

    doi: 10.3389/fimmu.2025.1531171

    Figure Lengend Snippet: Identification of the scFv anti-CD36 D11 (A) Strategy of panning of the ALTHEA Gold Libraries™ for identification of anti-CD36 scFvs. (B) Phage ELISA for 90 randomly selected clones from the third round of panning. Graph shows the signals generated by binding to rhCD36 (red) or protein L (ProL; brown). As control, we detected rhCD36 with the commercial antibody JC63.1 (blue). (C) Determination of structural integrity of purified scFv D11 by denaturing SDS-PAGE. R, reducing condition; NR, non-reducing condition; MW, molecular weight. (D, E) ELISA assay evaluating the binding of purified D11 (green) to protein L (D) or rhCD36 (E) . JC63.1 (blue) was used as a positive control and specificity was assessed by using the unrelated scFv P5E1A6 (black). (F) Representative competition ELISA (from two performed) evaluating the binding to rhCD36 captured by the reference antibody JC63.1 in presence of D11 (green) or the specificity control P5E1A6 (black). The signal generated in the absence of D11 is shown in red. (G) Number of clones identified at each stage of the discovery process. (G) was created with Biorender.com .

    Article Snippet: Nunc Maxisorb plates (Thermo Scientific, Cat. 278743) were coated with 50 µg/mL recombinant human CD36 (rhCD36) from Sino Biological (Cat. 10752-H08H) in PBS overnight at 4°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Clone Assay, Generated, Binding Assay, Control, Purification, SDS Page, Molecular Weight, Positive Control

    Binding of D11 to membrane CD36. (A) CD36 expression in macrophage-like THP-1 cells, as detected with the anti-CD36 FA6-152 (pink). The fluorescence generated by staining with the secondary antibody alone is shown in gray. (B) Concentration-response curve evaluating the binding of D11 to CD36 + macrophage-like THP-1 cells. Data from two independent experiments. Representative histograms are shown on the right. (C) CD36 expression in HepG2 cells. (D) Binding of D11 (green) to HepG2 cells and representative histograms (right). Data are representative of two independent experiments, each one including duplicates. (B, D) show the signals from the secondary antibody anti-His-tag PE (gray) and the unrelated scFv P5E1A6 (black).

    Journal: Frontiers in Immunology

    Article Title: Discovery and in vitro characterization of a human anti-CD36 scFv

    doi: 10.3389/fimmu.2025.1531171

    Figure Lengend Snippet: Binding of D11 to membrane CD36. (A) CD36 expression in macrophage-like THP-1 cells, as detected with the anti-CD36 FA6-152 (pink). The fluorescence generated by staining with the secondary antibody alone is shown in gray. (B) Concentration-response curve evaluating the binding of D11 to CD36 + macrophage-like THP-1 cells. Data from two independent experiments. Representative histograms are shown on the right. (C) CD36 expression in HepG2 cells. (D) Binding of D11 (green) to HepG2 cells and representative histograms (right). Data are representative of two independent experiments, each one including duplicates. (B, D) show the signals from the secondary antibody anti-His-tag PE (gray) and the unrelated scFv P5E1A6 (black).

    Article Snippet: Nunc Maxisorb plates (Thermo Scientific, Cat. 278743) were coated with 50 µg/mL recombinant human CD36 (rhCD36) from Sino Biological (Cat. 10752-H08H) in PBS overnight at 4°C.

    Techniques: Binding Assay, Membrane, Expressing, Fluorescence, Generated, Staining, Concentration Assay

    Effect of D11 in the uptake of CD36 ligands. (A) Analysis of oxLDL-DyLigth uptake in macrophage-like THP-1 cells. Cells were treated with D11 (green), JC63.1 (blue), or the unrelated P5E1A6 (black). Response was normalized against the MFI of untreated cells. Data from three independent experiments. (B) Representative histograms of the effect of the different treatments (100 µg/mL) on the uptake of oxLDL. (C) Effect of the listed antibodies (100 µg/mL) on the uptake of BODIPY-palmitate in HepG2 cells. Bars in the graph show the percentage of cells with BODIPY high fluorescence from three independent experiments (mean ± SEM). *p < 0.05; **p < 0.01, ns: not significant, Bonferroni’s multiple comparisons test. (D) Representative dot plots of the experiments reported in (C) .

    Journal: Frontiers in Immunology

    Article Title: Discovery and in vitro characterization of a human anti-CD36 scFv

    doi: 10.3389/fimmu.2025.1531171

    Figure Lengend Snippet: Effect of D11 in the uptake of CD36 ligands. (A) Analysis of oxLDL-DyLigth uptake in macrophage-like THP-1 cells. Cells were treated with D11 (green), JC63.1 (blue), or the unrelated P5E1A6 (black). Response was normalized against the MFI of untreated cells. Data from three independent experiments. (B) Representative histograms of the effect of the different treatments (100 µg/mL) on the uptake of oxLDL. (C) Effect of the listed antibodies (100 µg/mL) on the uptake of BODIPY-palmitate in HepG2 cells. Bars in the graph show the percentage of cells with BODIPY high fluorescence from three independent experiments (mean ± SEM). *p < 0.05; **p < 0.01, ns: not significant, Bonferroni’s multiple comparisons test. (D) Representative dot plots of the experiments reported in (C) .

    Article Snippet: Nunc Maxisorb plates (Thermo Scientific, Cat. 278743) were coated with 50 µg/mL recombinant human CD36 (rhCD36) from Sino Biological (Cat. 10752-H08H) in PBS overnight at 4°C.

    Techniques: Fluorescence

    Effect of D11 on the oxLDL-induced foam cell phenotype. (A) Effect of treatments on LD accumulation in macrophage-like THP-1 cells exposed to oxLDL (50 μg/ml) for 16-18 h. Graph shows the quantification (mean ± SEM) of oil red staining from two independent experiments. **p < 0.01; ****p < 0.0001, Bonferroni´s multiple comparisons test. (B) Representative micrographs of the experiments reported in (A) . (C-E) Effect of treatments on the relative expression of CD36 (C) , SRA1 (D) , and ACAT (E) . Graphs show the geometric mean ± geometric SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, ns: not significant, Bonferroni´s multiple comparisons test.

    Journal: Frontiers in Immunology

    Article Title: Discovery and in vitro characterization of a human anti-CD36 scFv

    doi: 10.3389/fimmu.2025.1531171

    Figure Lengend Snippet: Effect of D11 on the oxLDL-induced foam cell phenotype. (A) Effect of treatments on LD accumulation in macrophage-like THP-1 cells exposed to oxLDL (50 μg/ml) for 16-18 h. Graph shows the quantification (mean ± SEM) of oil red staining from two independent experiments. **p < 0.01; ****p < 0.0001, Bonferroni´s multiple comparisons test. (B) Representative micrographs of the experiments reported in (A) . (C-E) Effect of treatments on the relative expression of CD36 (C) , SRA1 (D) , and ACAT (E) . Graphs show the geometric mean ± geometric SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, ns: not significant, Bonferroni´s multiple comparisons test.

    Article Snippet: Nunc Maxisorb plates (Thermo Scientific, Cat. 278743) were coated with 50 µg/mL recombinant human CD36 (rhCD36) from Sino Biological (Cat. 10752-H08H) in PBS overnight at 4°C.

    Techniques: Staining, Expressing

    D11 impairs LD accumulation and clonogenicity on HepG2 cells. (A) Experimental protocol for the analysis of phenotypic changes induced by CD36 blockage. (B) Effect of treatments on LD accumulation. The bars in the graph (mean ± SEM) show the oil red-stained area from two independent experiments. **p < 0.01; ***p < 0.001; ****p < 0.0001, ns: not significant, Bonferroni´s multiple comparisons test. (C) Representative micrographs of the results presented in (B) . (D) Effect of treatments (100 µg/mL) on CD36 mRNA expression. Graph shows the geometric mean ± geometric SD from two independent experiments. *p < 0.05; **p < 0.01, ns: not significant, Bonferroni´s multiple comparisons test. (E) Experimental protocol for the evaluation of the effect of scFv D11 on the clonogenicity of HepG2 cells. (F) Effect of treatments on sphere formation efficiency (SFE). Data (mean ± SEM) from three independent experiments. *p < 0.05; ****p < 0.0001, ns: not significant, Bonferroni´s multiple comparisons test. (G) Representative micrographs of the results presented in (F) , with insets showing enlarged images of tumorspheres (scale=100 µm). (A, E) were created with Biorender.com .

    Journal: Frontiers in Immunology

    Article Title: Discovery and in vitro characterization of a human anti-CD36 scFv

    doi: 10.3389/fimmu.2025.1531171

    Figure Lengend Snippet: D11 impairs LD accumulation and clonogenicity on HepG2 cells. (A) Experimental protocol for the analysis of phenotypic changes induced by CD36 blockage. (B) Effect of treatments on LD accumulation. The bars in the graph (mean ± SEM) show the oil red-stained area from two independent experiments. **p < 0.01; ***p < 0.001; ****p < 0.0001, ns: not significant, Bonferroni´s multiple comparisons test. (C) Representative micrographs of the results presented in (B) . (D) Effect of treatments (100 µg/mL) on CD36 mRNA expression. Graph shows the geometric mean ± geometric SD from two independent experiments. *p < 0.05; **p < 0.01, ns: not significant, Bonferroni´s multiple comparisons test. (E) Experimental protocol for the evaluation of the effect of scFv D11 on the clonogenicity of HepG2 cells. (F) Effect of treatments on sphere formation efficiency (SFE). Data (mean ± SEM) from three independent experiments. *p < 0.05; ****p < 0.0001, ns: not significant, Bonferroni´s multiple comparisons test. (G) Representative micrographs of the results presented in (F) , with insets showing enlarged images of tumorspheres (scale=100 µm). (A, E) were created with Biorender.com .

    Article Snippet: Nunc Maxisorb plates (Thermo Scientific, Cat. 278743) were coated with 50 µg/mL recombinant human CD36 (rhCD36) from Sino Biological (Cat. 10752-H08H) in PBS overnight at 4°C.

    Techniques: Staining, Expressing

    Role of CD36 in the clinical prognosis and immune infiltration correlation in cutaneous melanoma: ( A ) Hallmark enrichment plot of CD36 generated with core cancer hallmark gene set ( n = 1574) shows that CD36 is associated with invasion and metastasis of melanoma as well as reprogramming energy metabolism ( p < 0.05). ( B ) Progression-free survival with CD36 expression in melanoma patients, using upper (red) and lower (blue) quartile data from the SKCM dataset. ( C ) Correlation of CD36 with CD163, CD14, CD209, and FAP in SKCM dataset ( n = 471). ( D ) CD36 positively correlates with the infiltration level of M2 macrophages in the SKCM-metastasis dataset ( n = 368). ( E ) Correlation between CD36 and infiltration level of endothelial cells in SKCM-metastasis dataset ( n = 368).

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: Role of CD36 in the clinical prognosis and immune infiltration correlation in cutaneous melanoma: ( A ) Hallmark enrichment plot of CD36 generated with core cancer hallmark gene set ( n = 1574) shows that CD36 is associated with invasion and metastasis of melanoma as well as reprogramming energy metabolism ( p < 0.05). ( B ) Progression-free survival with CD36 expression in melanoma patients, using upper (red) and lower (blue) quartile data from the SKCM dataset. ( C ) Correlation of CD36 with CD163, CD14, CD209, and FAP in SKCM dataset ( n = 471). ( D ) CD36 positively correlates with the infiltration level of M2 macrophages in the SKCM-metastasis dataset ( n = 368). ( E ) Correlation between CD36 and infiltration level of endothelial cells in SKCM-metastasis dataset ( n = 368).

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: Generated, Expressing

    Melanoma-derived EVs upregulate CD36 expression in the recipient cells. ( A ) Brief procedure for LEV and melanoma-cell-derived EV collection and characterization. Summarily, afflicted afferent lymph channels were surgically excised, and effluents of channels were utilized to collect LEVs through size-exclusion chromatography. Cell line culture-conditioned media was used to collect EVs by ultracentrifugation. All collected EV characterizations were carried out with NTA (see method in for more details). ( B ) LEVs collected from patients possess a significantly higher percentage of CD36 + EVs in total LEVs than control LEVs. ( C ) SKMEL28 and C32TG melanoma-cell-derived EVs increase expression of CD36 in HLEC (endothelial) and THP1 (monocytic) cells after 24 h of exposures. ( D ) Microscopic observation of CFSE-labeled-SKMEL28-derived EVs in HLEC cells (scale bar: 100 µm). ( E ) ImageStream analysis of CFSE-labeled-SKMEL28-derived EVs in THP1 cells (scale bar: 7 µm). ( F ) The box plot indicates the real-time PCR data by Cq values of CD36 and GAPDH gene expression in EVs collected from melanoma cell lines. Original images of ( A , C ) can be found in .

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: Melanoma-derived EVs upregulate CD36 expression in the recipient cells. ( A ) Brief procedure for LEV and melanoma-cell-derived EV collection and characterization. Summarily, afflicted afferent lymph channels were surgically excised, and effluents of channels were utilized to collect LEVs through size-exclusion chromatography. Cell line culture-conditioned media was used to collect EVs by ultracentrifugation. All collected EV characterizations were carried out with NTA (see method in for more details). ( B ) LEVs collected from patients possess a significantly higher percentage of CD36 + EVs in total LEVs than control LEVs. ( C ) SKMEL28 and C32TG melanoma-cell-derived EVs increase expression of CD36 in HLEC (endothelial) and THP1 (monocytic) cells after 24 h of exposures. ( D ) Microscopic observation of CFSE-labeled-SKMEL28-derived EVs in HLEC cells (scale bar: 100 µm). ( E ) ImageStream analysis of CFSE-labeled-SKMEL28-derived EVs in THP1 cells (scale bar: 7 µm). ( F ) The box plot indicates the real-time PCR data by Cq values of CD36 and GAPDH gene expression in EVs collected from melanoma cell lines. Original images of ( A , C ) can be found in .

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: Derivative Assay, Expressing, Size-exclusion Chromatography, Control, Labeling, Real-time Polymerase Chain Reaction

    Melanoma-derived EVs regulate M2-macrophage-like characteristics by upregulating CD36. ( A ) CD36 surface expression was increased upon EV exposure to cells compared to the control in M0 macrophages derived from THP1 cells. ( B ) Melanoma-derived EV exposure increases M2-macrophage-like characteristics by upregulating CD163 in PMA-pretreated THP1 cells. ( C ) EVs collected from CD36-silenced SKMEL28 cells decrease M2 macrophage characteristics in THP1-treated cells through CD36 downregulation.

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: Melanoma-derived EVs regulate M2-macrophage-like characteristics by upregulating CD36. ( A ) CD36 surface expression was increased upon EV exposure to cells compared to the control in M0 macrophages derived from THP1 cells. ( B ) Melanoma-derived EV exposure increases M2-macrophage-like characteristics by upregulating CD163 in PMA-pretreated THP1 cells. ( C ) EVs collected from CD36-silenced SKMEL28 cells decrease M2 macrophage characteristics in THP1-treated cells through CD36 downregulation.

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: Derivative Assay, Expressing, Control

    Spatial MxIF image analysis of SLN (−), SLN (+) tissue from patients, and LN tissue from control subjects. ( A ) Box plot showing the percentage of cells expressing a specific marker of all cells in a field of view (FOV) (center panel) and proportion of CD36+ cells per FOV in the three groups of samples. ( B ) Estimated probability of CD36 cells colocalized with other immune cells (within a 40-micron radius of a CD36 cell) in an analyzed panel of markers using spatial regression models.

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: Spatial MxIF image analysis of SLN (−), SLN (+) tissue from patients, and LN tissue from control subjects. ( A ) Box plot showing the percentage of cells expressing a specific marker of all cells in a field of view (FOV) (center panel) and proportion of CD36+ cells per FOV in the three groups of samples. ( B ) Estimated probability of CD36 cells colocalized with other immune cells (within a 40-micron radius of a CD36 cell) in an analyzed panel of markers using spatial regression models.

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: Control, Expressing, Marker

     CD36  cell colocalization spatial regression model results of melanoma SLN (−) compared to control LN.

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: CD36 cell colocalization spatial regression model results of melanoma SLN (−) compared to control LN.

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: Control

    Primary-melanoma-secreted EVs in lymphatic fluid enter the SLN via afferent lymphatic vessels. The SLN components, including macrophages and endothelial cells, receive these EVs to upregulate CD36, possibly through CD36 cargoes (mRNA and protein). The upregulation of CD36 in the targeted cells can mediate the immunosuppression mechanism to promote the tumor-promoting niche in the SLN.

    Journal: Biomolecules

    Article Title: Melanoma-Derived Extracellular Vesicles Induce CD36-Mediated Pre-Metastatic Niche

    doi: 10.3390/biom14070837

    Figure Lengend Snippet: Primary-melanoma-secreted EVs in lymphatic fluid enter the SLN via afferent lymphatic vessels. The SLN components, including macrophages and endothelial cells, receive these EVs to upregulate CD36, possibly through CD36 cargoes (mRNA and protein). The upregulation of CD36 in the targeted cells can mediate the immunosuppression mechanism to promote the tumor-promoting niche in the SLN.

    Article Snippet: Recombinant Human CD36 Protein was loaded for CD36 positive control (catalog: 10752-H08H, Sino Biological, Chesterbrook, PA, USA).

    Techniques: